The R-4 Flow Reactor Heater is designed to satisfy the precise needs of flow chemistry for drug discovery.
The R4 Flow Reactor Heater is designed to satisfy the precise needs of flow chemistry for drug discovery.
Precise temperature control. The true temperature of each reactor is measured,
4 Independently heated zones. The temperature of each reactor can be set independently in the range ambient to 150 C.
Accepts both column and tube type reactors. Reactors for either heterogeneous flow or homogeneous flow can be "plugged-in".
Rapid response. Rates of temperature change of up to 80 C / minute.
Excellent visibility. The novel design of the glass heat exchangers ensures the entire working volume of the reactor is visible at all times.
Remote control via RS232. Temperatures can be set by remote control using simple serial commands via RS232.
Compact size. A single R-4 offers 4 independently heated reactors within a small footprint. Multiple R-4s can be stacked.
Compact size. A single R4 offers 4 independently heated reactors within a small footprint. Multiple R-4s can be stacked.
The Vapourtec V-10 is rewriting the rules of solvent evaporation in drug discovery.
The Vapourtec V-10 is rewriting the rules of solvent evaporation in drug discovery.
The Vapourtec V10 Rapid Solvent Evaporator is rewriting the rules of solvent evaporation in drug discovery.
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Vapourtec Ltd is committed to the development and manufacture of synthesis and solvent evaporation systems that have a significant positive impact on the speed of development of new chemicals. Vapourtec evaporation systems achieve many times faster rates of evaporation when compared with traditional technologies (e.g. centrifugal evaporators). This speed differential is achieved through the adoption of a totally new approach to solvent evaporation that has been developed by Vapourtec and is proprietary to Vapourtec.
Among the many benefits of Vapourtec products is their design for easy integration with standard liquid handling robots. Frustrated by the slow traditional offline batch processes? Simple and rapid, Vapourtec products offer you the opportunity for compound synthesis and solvent removal online, NOW.
Vapourtec Ltd
Place Farm
Ingham
Suffolk
IP31 1NQ
UK
Tel: +44 (0) 1284 728659
Fax: +44 (0) 1284 728352
E-mail: info@vapourtec.com
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Vapourtec V-10. Applications examples
Case 1 - Evaporation of solvents in a general bench chemistry environment
Example: Post synthesis, 150 mg of compound is in solution in 12 ml of DMF in a 30 ml vial. The DMF
must be removed before moving onto either the next stage of synthesis or a purification operation.
Vapourtec solution: The V-10 is located within a fume hood, connected only to a standard electrical
supply. The chemist places the vial into the cradle, selects the evaporation method labelled high boiling
point and presses START. The V-10 automatically locates the vial against the vapourisation head and
evaporates the solvent under precisely controlled conditions. The V-10 detects when the solvent has
evaporated and dries the compound into a physical structure that can be readily re-dissolved. This
process is performed without manual intervention. Typically, the V-10 can complete this drying operation
in 10 minutes.
Alternatives: i) Use a rotary evaporator with the disadvantages of tying up the Chemist’s valuable time
and yielding the dry compound in a round bottom flask.
ii) Use a centrifugal evaporator which may take upwards of 2 hrs to deliver what the V-10
can achieve in just 10 minutes.
Case 2 – Open access drying of prep. HPLC fractions
Example: A mass directed open access HPLC system operating in the 50 to 100 mg scale is delivering
aqueous / Acetonitrile fractions of up to 40 ml into boiling tubes. The requirement is to provide the dried
compounds in pre-tared 20 ml vials in preparation for weighing and re-dissolving to a known
concentration.
Vapourtec solution: The V-10 is located within a fume hood, only connected to a standard electrical
supply. The chemist selects the clean operation; this process cleans with system solvent the sample
loop that is an integral part of the V-10. The chemist then selects prime to prime the sample loop. The
clean and prime operations together take about 30 seconds to complete. The pre-tared 20 ml vial is
placed into the V-10s cradle, the boiling tube is placed into the aspiration location, the recipe labelled
HPLC fraction is selected and the start button pressed. Operating from now on in an entirely automated
manner the 40 ml fraction is aspirated from the boiling tube by the V-10 and dried directly into the pre-tared
20 ml vial. Typically the V-10 can complete this operation (drying of 40 ml fraction) within 35
minutes.
Alternatives: i) Dry the complete fraction into the boiling tube using a centrifugal evaporator. This
operation may take 10 to 12 hours. Re-dissolve the compound which may require
immersion in an ultrasonic bath, transfer the solution into the pre-tared 20 ml vial and
place once again into a centrifugal evaporator to dry the compound. The second drying
operation is faster than the first but still the V-10 has completed in a little over half an hour
what the centrifugal evaporator has taken an elapsed time of two working days to
complete.
ii) Use a rotary evaporator which will tie up the Chemist’s time but will also require the dry
compound to be either scraped from the flask or if it is not a crystalline solid re-dissolved
and transferred into the pre-tared vial. Once transferred into the vial the compound may
need to be dried once again either in a centrifugal evaporator or using nitrogen
blowdown.
Duncan Guthrie Vapourtec Ltd
Case 3 – Open access drying of fractions following FLASH chromatography
Example: A UV directed multiple column FLASH chromatography system using a 50 gram cartridge is
collecting peaks into an array of forty 18 mm x 150 mm tubes located in racks on the bed of a fraction
collector. After analysis the chemist identifies that the peak of interest is contained in 120 ml of the
cyclohexane / ethyl acetate solution distributed across 6 of the 40 tubes. The requirement is to provide
the dried compound in pre-tared 30 ml vials in preparation for weighing.
Vapourtec solution: The V-10 is located within a fume hood, connected to a simple X, Y, Z pipetting
robot. A serial data connection between the X, Y, Z robot and the V-10 allows the two systems to
communicate using RS 232 protocols. The chemist places the rack containing the array of 40 tubes onto
the bed of the X, Y, Z robot. The 6 tubes containing the peak of interest are identified to the system via
the user interface. The pre-tared 30 ml vial is placed by hand into the V-10’s cradle, the FLASH fraction
recipe is selected and the START button is pressed. Operating from now on in an entirely automated
manner the 120 ml fraction is aspirated from the array of 6 tubes by the V-10. During the operation, the
X, Y, Z robot is used to position the aspiration nozzle in each of the 6 tubes in sequence. Once the total
fraction is aspirated and dried, the V-10 utilizes the wash station on the X, Y, Z robot to clean the
aspiration nozzle and the V-10’s sample loop. Typically the V-10 can complete this operation (drying the
120 ml fraction) within 25 minutes.
Alternatives: i) Decant the contents of the 6 tubes into a flask and dry the compound using a rotary
evaporator, transfer the contents into the pre-tared vial, a second evaporation process
using blowdown may be required. This procedure requires significant manual
intervention soaking up the Chemist’s valuable time.
ii) Locate the 30 ml vial under the nozzle of a nitrogen blowdown system. Dispense the
solution 15 ml at a time by hand into the vial. Leave to dry, then repeat the 15 ml
dispense until all the 120 ml fraction has been dried into the tube. This is a very slow
labour intensive approach.
Case 4 – Drying of dilute HPLC fractions for impurity analysis
Example: A mass directed reverse phase chromatography system is used to isolate impurities within
potential drug compounds. The process is producing a dilute fraction containing less than 1 mg of
impurity within 150 ml of water / acetonitrile. This fraction is collected in a 250 ml reagent bottle. The
impurity must be dried and transferred with minimal loss and no contamination into a 2.5 ml vial for
analysis.
Vapourtec solution: The V-10 is located within a fume hood, only connected to a standard electrical
supply. The chemist selects the clean operation; this process cleans with system solvent the sample
loop that is an integral part of the V-10. The chemist then selects prime to prime the sample loop. The
clean and prime operations together take about 30 seconds to complete. The 2.5 ml vial is placed into
the V-10s cradle, the reagent bottle is located ready for aspiration, the recipe labelled HPLC fraction is
selected and the start button pressed. Operating from now on in an entirely automated manner the 150
ml fraction is aspirated from the reagent bottle by the V-10 and dried directly into the 2.5 ml vial. The V-10
requires 10 hours to complete this operation.
Alternative: i) The only practical alternative is to undertake repeated drying processes using a rotary
evaporator. The impurity is dried, re-dissolved and transferred into successively smaller
flasks until the volume is sufficiently small to enable transfer into the 2.5 ml vial. This
process is incredibly time consuming and the potential for total loss or contamination of
the impurity is extremely high.
Duncan Guthrie Vapourtec Ltd
Performance comparisons
Time to evaporate 10ml of pure solvent @ 40 degC
Solvent Vapourtec
V- 10
Centrifugal
evaporation
Blow- down
evaporation
DMF 4 minutes 90 minutes N/ A
Water 8 minutes 140 minutes 240 minutes
Methanol 4 minutes 70 minutes 40 minutes
Cyclo- hexane 1 minute 40 minutes 20 minutes
The Vapourtec V- 10 is rewriting the rules of
solvent evaporation in drug discovery
Rapid solvent evaporator